![]() EstK has a unique substrate preference for p-nitrophenyl acetate and remains active at low temperatures. HiTrap ® Capto Q is a strong anion exchanger prepacked with BioProcess Capto media for screening, and small-scale protein purifications using ion exchange chromatography (IEX). Size exclusion Step: Superdex 200 Increase HiLoad Superdex 200 pg HiPrep Sephacryl S-300 HR. Optimum activity of EstK was at 40☌ at 4☌ the activity was ~50% of its maximum. Cytiva HiTrap Capto Q IEX Columns Print Share Cytiva HiTrap Capto Q IEX Columns Strong quaternary ammonium (Q) anion exchanger 240.50 - 675.50 Specifications. HiTrap Capto Q or HiScreen Capto Q (AIEX). Capto Q has a strong quaternary ammonium (Q) anion exchanger coupled to a chemically modified, high-flow agarose matrix. It had substrate specificity towards esters of short-chain fatty acids, particularly, p-nitrophenyl acetate. General description HiTrap Capto Q is a strong anion exchanger prepacked with BioProcess Capto media for screening, and small-scale protein purifications using ion exchange chromatography (IEX). EstK was purified to homogeneity using standard procedures. Prior to harvest, cells were treated with 0.2 M MgCl2 to precipitate lipopolysaccharides in the outer membranes, which otherwise form aggregates with the secreted enzymes. Hong, Seunghee Lee, ChangWoo Jang, Sei-HeonĪn extracellular esterase, EstK, was purified from the psychrotrophic bacterium Pseudomonas mandelii grown at 25☌. Purification and properties of an extracellular esterase from a cold-adapted Pseudomonas mandelii Purification and properties of an extracellular esterase from a cold-adapted Pseudomonas mandelii ![]()
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